A = Log 10 (I 0 /I). • 0. Controlled by the computer. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Supersedes 1. Published Dec 16, 2020 | Updated Nov 07, 2022. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. Luminometers and fluorimeters allow for readout of luminescence (luciferase readouts) or fluorescence from an entire well of a micro-well plate such as a 96- or 384-well plate. Multimodal plate reader: Yes: 25 mm: Yes—Max. Plus 384. We would like to show you a description here but the site won’t allow us. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . Place the microplate in the drawer. 50/plate. 2. The. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range. Reader Documentation Version No. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. Page 1 of 8 . guaranteed 25 fmol/200 µL fluorescein (125 pM), black 96-well plate; 1 second integration time 15 fmol/75 µL. and California)The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. 2. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. FlexIII. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. $14,995. Two high-energy xenon flash lamps: Reading capabilities. From the dropdown Menu, select Wellplates. This can increase throughput and efficiency, thus saving time and space in the lab. UV absorbance detection. Used. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 1 – 2. #R8195. with Quartz ABS Plastic. 000 ABS • Resolution: 0. x. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. This can increase throughput and efficiency, thus saving time and space in the lab. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Plate Storage Solutions for Liquid Handling Automation Remote Lab Management. Compare this item. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. 0025 OD from 0. The luminescence was measured in a SpectraMax. e 3370 Costar 96 Well. x. Cell viability and cytoxicity assays. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. They are also sealable using different types of adhesive mats foils or films. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. These include real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR,. 96-well Molecular Devices P/N 42-000-0117 HE™ High Efficiency microplates, 384-well Molecular Devices P/N 0200-5201. • High antibody-binding surface improves the overall performance in. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. Published Dec 16, 2020 | Updated Dec 16, 2020. 96-well) while others have flexibility to. Mix samples in microplate wells by user-programmable Automix feature. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. • 0. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. Microplate format. 96-well-plate-scan-time-2-colors-1-site-well. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. White 96-well plate Plate formats. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Yes—whole plate dispenser (96 or 384 well) Hamamatsu: FDSS7000EX: High throughput screening system: Yes (optional feature). EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Sample by eye within . On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). #C1500) Control standard endotoxin (ACC cat. No accessories are needed and SoftMax® Pro Software. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. Important things to consider when purchasing a multimode microplate reader. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data. 039. We would like to show you a description here but the site won’t allow us. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. Never order or replace filters to optimize an assay again!6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. 00. This robust system is capable of reading 96 wells, each with its own sample beam and reference detector. REF (SpectraMax. Plate and Setup Rack Options Screens with selection of up to four Methods shown. (96- or 384-well) simultaneously and with high temporal resolution. The procedures in this protocol support the 96-well reaction plate. 0, or. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. #E010) Pyroplate 96-well microplate (ACC cat. The opaque walls prevent stray light from being detected from neighbouring. Mode Plate Reader. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Reads various kinds of 96-wells plates, and is equipped with shaking function. M5. Microplate Spectrophotometers. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Recertification service. Align the two slots on the bottle holder with the two pegs on the rail. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. M2. * Provides sufficient reagent for 10 plates (96-, 384-well) R8128 FLIPR Membrane Potential Assay Kit Blue (Explorer) (10) vials Component A (blue)* (1) buffer bottle. 0A-Sunnyvale, CA-Made in USAWe offer a range of multiwell plates including 384-well, 96-well and 24-well plates with a selection of membrane types:. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human AT1R receptor. Dual injectors for kinetic assays. 300 µL of Detergent Compatible Bradford reagent was added to each well. For more sample throughput, the system can be easily integrated into a full robotic The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 190. Product Compare 0. Use standard plate unless you see your type listed. Wells of 96-well plates (NEST) were pre-coated with 100 μg/ml of collagen solution for 1 hour. (96- and 384-well with pipetting) 6-384 (96- and 384-well). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. $3,450. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical and. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. 5 ng per well. Published Dec 16, 2020 | Updated Dec 16, 2020. Instrument settings can. Gemini EM Reader scans. Or scan up to 384 wells at any time to determine the best assay conditions. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. Other technologies such as imaging. Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). style. Products : General, FilterMax F5 reader. 24-, 96-, 384-well plates and microwell strips; Manifold Channels: 96-well washing: 8-well (1x8) manifold, 2x8-well manifold, 12-well (1x12) manifold, 8-well short tube (1x8) manifold. When a. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Cell line development teams will realize savings on time and resources, as well as faster time-to-market by utilizing a single technology for measuring a critical quality attribute duringOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Our. Cayman Chemical. It also integrates with the powerful SoftMax Pro Software for. #3603) SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. Sort By: Show:. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. 000 to 2. 0% ± 0. Therefore you need to ask a physicist. Up to 96 well plates, there is often a good signal that can easily be detected, and 96 wells are measured comparatively quickly. measurements in compatible Molecular Devices® microplate readers. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Create custom fluorescence assays without having to purchase expensive filter sets. 76358-624. Plate. Uncontrolled Copy for Training and Reference Purposes Only . Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). absorbance microplate reader GEA. BioTek LogPhase 600 microbiology reader measures microbial growth curves in up to four standard 96-well microplates at a time. Molecular Devices. 500 USD. This reader can also be used as a standard. Quantitative, whole well cell confluence for every well. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. 6-384 (96- and 384-well with pipetting) 96-well microplates. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. 1 mL/well of media. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. FlexIII. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Optional 16-way manifold. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Single or dual wavelength read, kinetic readings, automix, endpoint, and blank pattern, curve fits included with PC Software. QC tools and IQOQ services. The CellTiter-Glo assay was used to measure ATP. The eight-channel system delivers high precision and speed when reading 96-well microplates for single endpoint measurements and kinetic assay data gathered over time. When the user applies a set temperature of 37°C, the top is heated to 37. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 1. The ELx808 TM Reader seamlessly interfaces with Lonza's WinKQCL TM Endotoxin Detection and Analysis Software. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. 1% in white 96- and < 0. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Range of 96, 384-well SBS microplates . Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. The Best Microplate Readers According to our Product Reviewers (1) Write a Review. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. To test the optical clarity of the various microplates at low wavelengths, one well of each plate was filled with 100 μL (96-well) or 20 μL (384-well) distilled water, and its. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible. Description. Usage/Application: Lab. ZERO BIAS - scores, article reviews, protocol conditions and moreThe VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. (Molecular Devices cat. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. 5 fg per well to 0. HeLa. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. *For Corning UV plates, mean values at 260 nm ranged from 0. The four standard filters – 405, 450, 492, 620 nm – allow for a wide variety of ELISAs such as PNPP, ABTS, OPD and TMB. 1% in white 96- and < 0. Materials. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Working in. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. x. $3,207. Products : Ionworks HT, Ionworks Quattro. • Photometric range: 0. Intuitive graphical user interface makes each assay extremely easy to set. Expand Microplates and select the newly created plate. 340 PC384. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. 0200-6263. Can read 96 well plates (optional 384 well). With more than 40 years of plate reader expertise and life science research, we’ve amassed an extensive. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. perfect for kinetic readings involving growth curves at 5 seconds per plate. This reader's dual monochromators for. Shaking: Linear, orbital, and double orbital. An entire 96-well plate can be processed in less than six seconds. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Figure 2-23: Bottle holder To install the bottle holder: 1. Plate types Flat and round 96-well plates Data output Export to PC Detection system Single channel silicon photodiode Reading speed 25 seconds Software SoftMax Pro Software Shaking None Temperature control None Linearity 0. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Three low volume 24-well bottom slides. Linear, Orbital Slow, and Orbital Fast. x. SoftMax Pro GxP Software version 5. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. Top and bottom reads are. Instrument settings can. White 96-well platePlate formats. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 46Click the Read button in the SoftMax Pro 8-channel (96) for FlexStation 3 Reader • 8-channel pipettor • (10) racks of 96-well, FlexStation Pipet Tips (Black) • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettor • (10) racks of 384-well, FLIPR® Tetra Pipet Tips (Clear) • 384-well yellow plate 0200-6183 Consumables ELISA Plate Readers / Microplate Readers. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. the Gemini XPS reader. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. xThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Anticancer drug assay was also conducted using conventional 96-well plates as controls. 0% ± 0. Make sure toPlate reader instructions Computer. HeLa. For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro densitometry of TLC plates and electrophoresis gels. It features a modular design that allows users to add or change detection cartridges according to their needs. Eight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. Molecular Devices' microplate readers. The. x. (one set per assay) to clear 96-well microplates. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. Compact designs make these instruments. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. µl) was added to each well and incubated at 37°C for 1 h. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Step 3: Click “Settings” and a screen will appear (Figure 1). 1c shows a cover designed to perfuse 8 wells in series on a standard 96-well microplate, as well as a cover for the entire standard 96-well microplate. Molecular Devices LLC 96 well plate reader 96 Well Plate Reader, supplied by Molecular Devices LLC, used in various techniques. Lucid Scientific. 01. Select up to 3 products. Description Bottom Volume Qty/Pk Qty/Cs 3635 96 Well UV-Plate Flat 370 µL 2550per well of this semi-solid mixture was pipetted into 6-well plates. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm) with 4 filter choices included at time of purchase. VIS absorbance detection. A barcode reader accessory can be purchased with theWe would like to show you a description here but the site won’t allow us. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 20 attomol ATP per well. One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. Versa Max. Part of a plate can be selected so that only a selected area of the plate. Max, FlexStation, Gemini, and earlier instruments as well as import and analysis of data files from FLIPR and Analyst instruments. Dimensions: 18. 5% at 1. 5 µL of each sample was pipetted into a 96-well plate for PCR. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Instrument pricing. x. • Easy and gentle immobilization of biotin. SpectraDrop High-Throughput Screening (HTS) Kit. Conventional technique: subjective, time-consuming. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. BioTek Instruments Synergy Neo2 Multi-Mode Reader Fluorescence Measuring Equipment. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Please click on the links below to view the Microplate Reader Comparison Tables. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . 7°C (gradient heating design). SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. The Absorbance 96 Plate Reader is designed to run everyday assays with ease and speed. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Molecular Devices (UK) Ltd. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. Corning. to each well. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The IC50s by cell rounding and LysoTracker. Selects either 96-well or 384-well plate for a stand-alone read. Materials. . Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Washers configured for both 96- and 384-well plates. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Related Products: 96 Well Plate Reader. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). Fluorescence Microplate Reader Comparison Chart. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl. a white 96-well half-area microplate (Corning cat. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Microplate Reader Ordering information Item Description Part number FLIPR Membrane. To help you get the best results from your Molecular Devices® plate readers it is essential that you choose the right plate. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The system can perform readings. Infinite possibilities. No. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. It offers extremely low absorbance background, excellent. With theMolecular DevicesWe designed and fabricated different sized microfluidic covers, matched to the desired experimental conditions: Fig. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. They can be read by most microplate readers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. SoftMax Pro GxP Software version 5. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. 20 µL (96-well plates) or 10 µL (384. Two different agonists, acetylcholine and carbachol, were tested. minutes and begin reading microplates with seven mouse clicks. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. For biochemical assays, the washer removes unbound material and unreacted reagents. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. variety of. 5in (46. Well-to-WellPrecision RFULinearity RelativeFluorescenceUnit(RFU)ScaleRatio(GeminiEM,XPS,SpectraMaxM2-M5e, FlexStation3only). SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Published Dec 16, 2020 | Updated Dec 06, 2021. 9. the absorbance at 340 nm at 37 °C was measured every 30 s using SpectraMax M5 Microplate. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. 1. Plate SpectraMaxiD3,iD5,i3x,i3,M2,M2e,M3,M4,M5,M5e,Plus. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. 984. Automated live cell imaging – cell counting and confluence. These are the default kind of cuvette & 96 well plate stocked by the lidstrom lab as of 11/2013. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume differences that may.